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Schedule of Topics for Biol. 326
Electron Microscope Techniques in Biology

Instructor: Robert J. Schmitz
Biol. 326 Home  |  Biol. 326 Course Policies

This list of topics is meant to an guide to the subjects that will be studied in the course. As the course progresses this list will vary slightly depending on the needs of the students enrolled.  

week of semester Topics Rental Text reading

preclass

 Please read Chapter 1 and Chapter 21 of Bozzola prior to comming to class Bozz Chapter 1, 21
WK 2  Lecture: Introduction to the process of specimen preparation for TEM
 Tues. Lab: Introduction to the lab and lab safety, glass knife making, block trimming
 Thurs. Lab: Practice knife making and block trimming
Bozz Chapter 3
WK 3  Lecture: The Ultramicrotome
 Tues.Lab: Using the Ultrmicrotome
 Thurs. Lab: Thick sections
Bozz Chapter 4
WK 4  Lecture: Introduction to the Transmission Electron Microscope
 Tues. Lab: TEM Demonstration
 Thurs. Lab: Block Trimming, Thick sections
Bozz Chapter 6
WK 5  Lecture: Making thin sections and staining grids
 Tues. Lab: Making thin sections
 Thurs. Lab: Sectioning
Bozz Chapter 5
WK 6  Lecture: Specimen Fixation and Embedding
 Tues. Lab: Plan fixation and Embedding procedure, Demonstrate Grid staining
 Thurs. Lab: Sectioning
Bozz Chapter 2
WK 7  Lecture and Tues. Lab:  Fix specimens

 Thurs. Lab: Embed specimens (will carry over into Friday and Saturday)

WK 8  Lecture: Theory and use of the Transmission Electron Microscope
 Tues. Lab: Using the Electron Microscope
 Thurs. Lab: Sectioning
Bozz Chapter 6
WK 9  Lecture: Principles of Cell Ultrastructure
 Tues. Lab: Using the Electron Microscope
 Thurs. Lab: Sectioning
Bozz Chapter 20
Cross and Mercer
WK 10  Lecture: Artifacts
 Tues. Lab: Using the Electron Microscope
 Thurs. Lab: Sectioning (or fixing and Embedding plant tissues)
Bozz Chapter 19
WK 11  Lecture: Producing the Electron Micrograph
 Tues. Lab: Using the Electron Microscope
 Thurs. Lab: Sectioning
Bozz Chapter 8, 18
WK 12  
 Tues. Lab: Using the Electron Microscope or making prints
 Thurs. Lab: Sectioning

WK 13  
 Tues. Lab: Using the Electron Microscope or making prints
 Thurs. Lab: Sectioning Ends

WK 14  
 Tues. Lab:  Using the Electron Microscope or making prints
 Thurs. Lab: Using the Electron Microscope or making prints

WK 15  Lecture: Exam
 Tues. Lab: Using the Electron Microscope or making prints
 Thurs. Lab: Micrograph Portfolio Due and Lab Checkout

Finals WK  Meet to discuss scores, evaluate portfolio and determine grade